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Pure agar is used to make culturing media for biology experiments. Provided as a dehydrated powder. The contents of this bottle are sufficient to make enough culturing media to make up about fifty 90mm Petri dishes or several hundred slant culturing tubes or test tubes. This agar is the non-nutrient form. If you need nutrient agar, simply make up the agar solution with dilute chicken or beef broth instead of pure water.
Make up the agar solution by adding about one level teaspoon of the agar powder to 100 mL of boiling water (or water/broth solution) and stirring until the solution is an even consistency. You must sterilize the agar before use. To do so, use one of the following procedures:
• If you are using sterile plastic Petri dishes or culture tubes, sterilize the agar solution itself by putting the vessel that contains the agar solution in a pressure cooker for 15 minutes at 15 pounds pressure (121 ?C/ 250 ?F). Be careful to preserve the sterility of the Petri dishes or culture tubes as you fill them with agar solution.
• If you are using glass Petri dishes or culture tubes, fill the Petri dishes or culture tubes and then sterilize them in a pressure cooker for 15 minutes at 15 pounds of pressure.
Low hazard for usual laboratory handling.
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